Purification of Penicillin - Induced Penicillinase of Bacillus cereus NRRL 569
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چکیده
We wish to express our thanks to Dr Melvin Cohn for much valuable advice during the preliminary work on methods of purification, and one of us (M.R.P.) is greatly indebted to Dr Jacques Monod of the Institut Pasteur, Paris, for the hospitality of his laboratory in which much of the earlier work on isolation ofpenicillinase was carried out. We are also grateful to Dr T. S. Work for help in the chromatographic estimation of amino acids. The photographs were taken by Mr M. R. Young. The Perkin-Elmer Tiselius apparatus used for electrophoresis analysis was purchased by meauIs of a grant from Eli Lilly and Co.
منابع مشابه
The cell-bound penicillinase of Bacillus cereus.
Only 30-50 yo of the cell-bound penicillinase of Bacillus cereus NRRL 569 is neutralized by antiserum prepared against the exo-penicillinase. The unneutralizable fraction is not decreased by cell disintegration which liberates a proportion of the cell-bound enzyme into solution. Absence of neutralization cannot therefore be explained by the existence of a mechanical barrier which prevents acces...
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Imsande, John (Western Reserve University, Cleveland, Ohio). New assay for penicillinase and some results on penicillinase induction. J. Bacteriol. 89:1322-1327. 1965.-A rapid, sensitive, and reliable assay of penicillinase activity is described. Studies conducted on the induction of penicillinase, with use of the new assay, show that actinomycin D inhibits growth and the induced synthesis of p...
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